This research used samples and/or data provided by the Ocean Drilling Program (ODP). ODP is sponsored by the U.S. National Science Foundation (NSF) and participating countries under management of Joint Oceanographic Institutions (JOI), Inc. This research was partially supported by the Taiwan National Science Council (MZ). We are grateful to Dr. Pinxian Wang and Simon Brassell for discussion and suggestions about the evolution of temperature control on alkenone unsaturation. We'd like to express our appreciation to Dr. Warren Prell and Dr. Timothy Herbert for their constructive and detailed reviews that helped to improve this manuscript.