Table T10. Aerobic and anaerobic cultivation media.

Table T10. Aerobic and anaerobic cultivation media.

Media components

Aerobic

Anaerobic

Peptone 3 g 3 g

Yeast extract 1 g 1 g

Glucose 3 g 3 g

Elemental sulfur 50 mL 50 mL

Trace elements components 10 mL 10 mL

Resazurin solution (0.2 g/L) — 5 mL

Cysteine-HCl — 10 mL

Artificial seawater 1 L 1 L

Media components	Aerobic	Anaerobic
Peptone	3 g	3 g
Yeast extract	1 g	1 g
Glucose	3 g	3 g
Elemental sulfur	50 mL	50 mL
Trace elements components	10 mL	10 mL
Resazurin solution (0.2 g/L)	—	5 mL
Cysteine-HCl	—	10 mL
Artificial seawater	1 L	1 L

Notes: Elemental sulfur was sterilized by steaming for 1 hr at 100°C on three successive days. Trace element components (1 L); CuSO₄ (0.01 g), ZnSO₄ (0.1 g), CoCl₂ (0.005 g), MnCl₂ (0.2 g), Na₂MoO₄ (0.1 g), KI (0.05 g), LiCl (0.05 g), H₂WO₄ (0.005 g) NaSO₄ (0.005 g), BaCl₂ (0.005 g).