Figure F11. Gradient culture tubes were prepared by fixing 0.5 mL of a monomer medium (Mono) on the bottom with agar. Then, substrate-free mineral medium with agar was added as a spacer, a sediment piece was positioned, and the tube was filled with mineral medium and gassed with nitrogen on ice. MM = marine salts medium.

Figure F11. Gradient culture tubes were prepared by fixing 0.5 mL of a monomer medium (Mono) on the bottom with agar. Then, substrate-free mineral medium with agar was added as a spacer, a sediment piece was positioned, and the tube was filled with mineral medium and gassed with nitrogen on ice. MM = marine salts medium.