Figure F6. Diagram of WRC sectioning for microbiology (MBIO). After taking syringe samples on the catwalk, two complete, sequential, 1.5-m sections were rapidly taken to the cold room (4°-10°C). Typically, Section 3, if not visibly disturbed, was subsampled after cleaning and alcohol wiping. The first cut (bold line) was done with the ODP cutter and separated the section into a portion to be further sectioned cleanly and anoxically in a cutting rig and one to be cut with the ODP cutter. Both sections were handled simultaneously and in the order shown. A sample for interstitial water (IW) was the first section taken and was immediately given to the geochemists for processing (unless already taken on the catwalk from the adjacent section end). The next section was for slurries, if required (nonroutine subsampling). This was rapidly cut, and at the middle cut of the liner the core was broken so that the broken surfaces were pointing downward after breaking (dotted line). The middles of the two broken parts were then immediately subsampled. AODC = acridine orange direct count, PFC = perfluorocarbon tracer, WRC = whole-round core, FISH = fluorescence in situ hybridization, DNA = deoxyribonucleic acid, ATP = adenosine triphosphate, SRR = sulfate reduction rates, DIFF = diffusion experiments, AOM = anaerobic oxidation of methane, MPN = most probable number. CHNS = carbon, hydrogen, nitrogen, sulfur analyses.

Figure F6. Diagram of WRC sectioning for microbiology (MBIO). After taking syringe samples on the catwalk, two complete, sequential, 1.5-m sections were rapidly taken to the cold room (4°-10°C). Typically, Section 3, if not visibly disturbed, was subsampled after cleaning and alcohol wiping. The first cut (bold line) was done with the ODP cutter and separated the section into a portion to be further sectioned cleanly and anoxically in a cutting rig and one to be cut with the ODP cutter. Both sections were handled simultaneously and in the order shown. A sample for interstitial water (IW) was the first section taken and was immediately given to the geochemists for processing (unless already taken on the catwalk from the adjacent section end). The next section was for slurries, if required (nonroutine subsampling). This was rapidly cut, and at the middle cut of the liner the core was broken so that the broken surfaces were pointing downward after breaking (dotted line). The middles of the two broken parts were then immediately subsampled. AODC = acridine orange direct count, PFC = perfluorocarbon tracer, WRC = whole-round core, FISH = fluorescence in situ hybridization, DNA = deoxyribonucleic acid, ATP = adenosine triphosphate, SRR = sulfate reduction rates, DIFF = diffusion experiments, AOM = anaerobic oxidation of methane, MPN = most probable number. CHNS = carbon, hydrogen, nitrogen, sulfur analyses.