Figure F8. "Bucket" for clean and anoxic subsampling from WRC segments into sterile syringes with the Luer end removed. A 5-cm WRC segment is placed in the middle of the alcohol wiped and flamed bucket in which a steady nitrogen flow maintains anoxic conditions. The uppermost 1 cm is pushed out of the core liner and is cleanly removed. Subcores are then taken from the central part with sterile, cut-off syringes that are subsequently capped with sterile stoppers as shown.

Figure F8. "Bucket" for clean and anoxic subsampling from WRC segments into sterile syringes with the Luer end removed. A 5-cm WRC segment is placed in the middle of the alcohol wiped and flamed bucket in which a steady nitrogen flow maintains anoxic conditions. The uppermost 1 cm is pushed out of the core liner and is cleanly removed. Subcores are then taken from the central part with sterile, cut-off syringes that are subsequently capped with sterile stoppers as shown.