|
Component
|
Concentration
|
|---|---|
| Distilled water | 1000 mL/L |
| NaCl | 24.32 g/L |
| MgCl2·6H2O | 10 g/L |
| CaCl2·2H2O | 1.5 g/L |
| KCl | 0.66 g/L |
| Na2SO4 | 4.0 g/L |
| Trace element solution (SL 10)* | 1 mL/L |
| KBr (0.84 M) | 1 mL/L |
| H2BO2 (0.4 M) | 1 mL/L |
| SrCl2 (0.15 M) | 1 mL/L |
| NH2Cl (0.4 M) | 1 mL/L |
| KH2PO4 (0.04 M) | 1 mL/L |
| NaF (0.07 M) | 1 mL/L |
| Na2SeO3 (10-7 M)† | 1 mL/L |
| Na2WoO4 (10-7 M)† | 1 mL/L |
| Autoclave, cool under N2, then add the following from sterile stock solutions: | |
| NaHCO3 (1 M) | 30 mL/L |
| Vitamin solution‡ | 2 mL/L |
| FeCl2 (1 M) in 0.1-M HCl | 0.5 mL/L |
| Na2S (1 M) | 1.2 mL/L |
Notes: Adjust pH to 7.2-7.4 with sterile 1-M HCl or sterile 1-M Na2CO3. * = Widdel et al. (1983), † = Widdel (1980). ‡ = mixture of 10 vitamins, concentrated fivefold (after Balch et al., 1979).