Table T5. Media used for cultivations at 25°, 50°, and 80°C.
Medium
Components
Concentration
(% wt/vol)
Comments
201-1: Heterotrophic sulfur reducer medium Yeast extract 0.1 Prepare in MJ under N2 headspace

Peptone 0.1

Glucose 0.05

Na2S 0.05

Elemental sulfur 3

Wolfe's vitamin solution*

201-2: Heterotrophic Fe(III) reducer medium Yeast extract 0.1 Prepare in MJ under N2 headspace

Peptone 0.1

Glucose 0.05

Na2S 0.05

Ferrihydrite 1

Magnetite 1

Wolfe's vitamin solution*

201-3: Heterotrophic nitrate reducer medium Yeast extract 0.1 Prepare in MJ under N2 headspace

Peptone 0.1

Glucose 0.05

Na2SO4 0.05

NaNO3 1

Wolfe's vitamin solution*

201-4: Heterotrophic sulfate reducer medium Sodium lactate 0.05 Prepare in MJ under N2 headspace

Sodium acetate 0.05

Sodium formate 0.05

Sodium fumarate 0.05

Glucose 0.05

Na2SO4 0.1

Na2S 0.05

Cystein-HCl 0.05

Wolfe's vitamin solution*

201-5: Autotrophic H2-oxidizing sulfate reducer medium NaHCO3 0.15 Prepare in MJ under 80% H2 + 20% CO2 headspace
Na2SO4 0.1

Na2S 0.05

Cystein-HCl 0.05

Wolfe's vitamin solution* 0.15




201-6: Autotrophic HS-/S2O32-/S0 oxidizing Fe(III) or nitrate reducer medium NaHCO3 0.15 Prepare in MJ under 70% N2 + 30% CO2 headspace
Na2S2O3 0.1

Elemental sulfur 3

Ferrihydrite 0.05

NaNO3 0.05

Na2S 0.025

Wolfe's vitamin solution*

201-7: Micro-aerobic, autotrophic HS-/S2O32-/S0 oxidizing Fe(III)/NO3- reducer medium NaHCO3 15 Prepare in MJ under 68.5% N2 + 28.5% CO2 + 3% O2 headspace
Na2S2O3 0.1

Elemental sulfur 3

Ferrihydrite 0.05

NaNO3 0.05

Na2S 0.025

Wolfe's vitamin solution*

201-8: Methanogen medium NaHCO3 0.15 Prepare in MJ under 80% H2 + 20% CO2 headspace

Sodium acetate 0.05

Sodium formate 0.05

Methanol 0.05

Na2S 0.05

Cystein-HCl 0.05

Magnetite 0.05

Wolfe's vitamin solution*

201-9: Autotrophic NH4 + oxidizing Fe(III)/NO3- reducer medium NaHCO3 0.15 Prepare in MJ under 70% N2 + 30% CO2 headspace
NH4Cl 0.1

Ferrihydrite 0.1

NaNO3 0.1

CuSO4 0.05

Wolfe's vitamin solution*

201-10: Micro-aerobic, autotrophic NH4+ oxidizing Fe(III)/NO3- reducer medium NaHCO3 0.15 Prepare in MJ under 68.5% N2 + 28.5% CO2 + 3% O2 headspace
NH4Cl 0.1

Ferrihydrite 0.1

NaNO3 0.1

CuSO4 0.05

Wolfe's vitamin solution*

201-11: Extremely halophilic archaea (genus Haloarcula) medium NaCl 20.8 Prepare in MJ under air headspace; pH (25°C) = 7.2
Yeast extract 1.0

MgSO4·7H2O 4.7

CaCl2 0.05

MnCl2 0.013

Wolfe's vitamin solution*

Notes: MJ = artificial seawater solution (see Table T6). * = added at 1 mL/L; Freier et al., 1988.