Table T8. MSSM used as the basis for 60°C cultivations.

Table T8. MSSM used as the basis for 60°C cultivations.


Component	Concentration
Sigma sea salt	40. g/L
Na₂HPO₄·H₂O	2. mM/L
Modified (Ni, W) Wolfe's mineral	0.25 mL/L
Wolfe's vitamin solution*	5. mL/L
Yeast extract	0.5%/L
NH₄Cl	0.1%/L
Na₂CO₃	25. mM/L

Notes: MSSM = marine sea-salts media. Prepare solution in deionized, distilled water. * = Freier et al., 1988. pH (25°C) was adjusted to 8.5 and 10.0, respectively, before autoclaving. The pH after autoclaving and at 60°C is ~0.5 to 1.2 pH units (depending on media supplements) more acidic.