Figure F4. Phylogenetic tree of bacterial 16S rRNA gene sequences obtained from libraries prepared after amplification of DNA extracted from mixed cultures incubated for either 3 days or 7 months. Cloned 16S rDNA sequences are shown in bold text and the GenBank accession numbers for sequences obtained from the NCBI database are shown in parentheses. At day 3, most sequences are from Halomonas spp. and Vibrio spp. At 7 months, most clones belonged to the Vibrio spp.; however, some were found from Shewanella spp. Phylogram was made using neighbor joining method, with 100 bootstrap replicates.