|
Solution
|
Components
|
Concentration
|
Comments
|
|---|---|---|---|
| 10x PBS solution | NaCl | 40.0 g |
|
| KCl | 1.0 g |
|
|
|
|
KH2PO4 | 1.0 g |
|
|
|
Na2HPO4·2H2O | 5.75 g | Fill to 1000 mL with deionized water |
|
|
|
|
Adjust pH to 7.3 |
|
|
|
|
Autoclave |
| 4% Formaldehyde/PBS buffer | Deionized H2O | 90 mL | Heat to 60°C in fume cupboard |
| 2-M NaOH | 2 drops |
|
|
|
|
Paraformaldehyde | 4 g | Stir 2 min |
|
|
10x PBS solution | 10 mL | Cool on ice; adjust to pH 7.3 |
|
|
|
|
Filter through 0.45-µm filter |
Notes: This solution must be prepared fresh or kept frozen. PBS = phosphate buffered saline.