Based on the equation trend line from Pokrovsky and Schott's (2000b) data of olivine dissolution rates at 25°C and changing pH (Fig. AF1), an approximate dissolution rate of olivine was extrapolated at 25°C and pH 7.5 and 9.5 (Table AT6).
The moles of olivine that should have dissolved at each of the time points when ICP-MS analysis was used (Tables T6, T7) was calculated with the rates from Table AT6, the BET analysis SSA (Table AT3; see "Appendix A"), and the time converted to seconds. A step-by-step layout of how the values shown in Table T6 were calculated is in Table AT7. Table T7 was calculated the exact same way but over slightly different lengths of time.
Based on Oelkers (2001) data of changing temperature with a constant pH of 2.0, a trend line was created to extrapolate dissolution rates for 4°C and 15°C. However, the data yield negative dissolution rates for both temperatures. Because of this, the lower temperature dissolution rates were not used to calculate an estimate of abiotic olivine dissolution within the enrichment cultures and controls.