1. Dilute the perfluorocarbon tracer in methanol in the following dilution series:
- Add 0.1 mL PFT to 0.9 mL of methanol = 10-1
- Add 0.1 mL of the 10-1 dilution to 9.9 mL of methanol = 10-3
- Add 0.1 mL of the 10-3 dilution to 9.9 mL of methanol = 10-5
- Add 0.1 mL of the 10-5 dilution to 9.9 mL of methanol = 10-7
- Add 0.1 mL of the 10-7 dilution to 9.9 mL of methanol = 10-9
2. Transfer 10 µL of the 10-3, 10-5, 10-7, and 10-9 dilutions into duplicate 20-mL-capacity headspace vials and seal.3. Heat vials and gas-tight syringes (70°C).
4. Inject either 5.0 or 0.5 mL from each vial into the gas chromatograph.
5. Integrate the peak area for each injection and plot peak area against PFT(g) and perform a regression analysis to determine the slope. Table AT1 was constructed assuming the headspace vial capacity is 20 mL and the density of the PFT is 1.76 g/mL using the formula
Example: (5.0 mL of the 10-7 dilution)
