MATERIAL AND METHOD

Leg 178 Samples

During Leg 178 we cored nine sites on the continental rise (Sites 1095, 1096, and 1101), continental shelf (Sites 1097, 1100, 1102, and 1103), and in Palmer Deep (Sites 1098 and 1099), along the Pacific margin of the Antarctic Peninsula (Fig. F1; Table T1). The samples used in this study were taken from Holes 1095A, 1095B, 1097A, 1100D, and 1103A. Sample preparation techniques are described in Winter and Iwai (Chap. 29, this volume). Observations and photomicroscopy of diatoms were carried out using a Zeiss Axioskope microscope with a differential interference contrast (DIC) condenser at magnifications of 400x, 630x, and 1000x. Diatom species occurrence in all holes at Site 1095 (where the major diatom occurrences were found) is given in table T3 of Winter and Iwai, Chap. 29, this volume. Occurrences at Sites 1097, 1101, and 1103 are given in Tables T2, T3, and T4, respectively, of this data report.

Digital Imaging

Photomicrographs of diatoms were taken using a digital camera system with the Zeiss DIC Axioskope microscope. A digital color camera, Fuji HC-300Z, was attached to the microscope with a Nikon relay lens (4x). The HC-300Z has a single 2/3-in square chip and 8-bit RGB color transfer method. The exposure time ranged between 1/2000 and 1/4 s. High-quality images at a resolution of 1280 x 1000 pixels were recorded directly onto Zip media as JPEG files. Pictures were processed with Adobe Photoshop and Microsoft Powerpoint. Brightness and contrast were adjusted in order to homogenize each image, but no special procedure was performed.

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